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Title: Transcription profiling by array of zebrafish larvae exposed to 3g and 5g hyper gravity      
dateReleased:
02-27-2015
description:
The general objective of the study was to determine modulation of gene expression by environmental factors, with a special emphasis on bone formation. For this reason, the specific period of treatment was chosen between 5-6 days post-fertilization (dpf), when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a Large Diameter Centrifuge and brought to a gravitational force of 3g or 5g for 24 hours. We show that this treatment causes a clear increase of bone formation, as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red, by morphometric analysis of the resulting images and by gene expression studies of selected genes. Thus, a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3289
refinement:
raw
dateSubmitted:
10-21-2010
keywords:
functional genomics
dateModified:
02-02-2015
availability:
available
types:
gene expression
name:
Danio rerio
name:
stimulus or stress design
ID:
A-GEOD-10816
name:
Agilent-024129 Dano rerio 44K
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3289/E-MTAB-3289.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3289/E-MTAB-3289.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress