Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: The effect of the autoimmune regulator (Aire) gene on the transcriptional expression profile of 3.10 mouse medullary thymic epithelial cell line      
dateReleased:
12-31-2015
description:
Medullary thymic epithelial cells (mTECs) contribute to self-tolerance through the ectopic expression of peripheral tissue antigens (PTAs) in the thymus. PTA expression in mTECs is largely dependent on the autoimmune regulator (Aire) gene. Here we used a Mus musculus mTEC cell line (3.10 mTEC line, which constitutively express Aire in culture) to knockdown Aire gene by means of siRNA transfection. Aire knockdown was confirmed by means of qRT-PCR and RNA-FISH (for Aire mRNA levels), immunofluorescence and western blot (for AIRE protein levels).The Agilent oligo microarrays were used to determine the large scale transcriptional expression profiles of control or Aire-knockdown 3.10 mTECs.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3879
refinement:
raw
dateSubmitted:
02-25-2015
keywords:
functional genomics
dateModified:
09-11-2015
creators:
Geraldo Passos
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-MEXP-2114
name:
Agilent SurePrint G3 Mouse GE 8x60K Microarray 028005 ( 85 cols x 532 rows )
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3879/E-MTAB-3879.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3879/E-MTAB-3879.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.