biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | Transcriptional profiling by array of Solenopsis invicta (fire ant) newly mated queens to identify genes and gene pathways responding to infection by SINV viruses
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| dateReleased: |
01-04-2016
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| description: |
We performed a whole-genome transcriptomic analysis of pleometrotic queens infected by SINV-1, SINV-2 or co-infected with both viruses, to characterize patterns of gene expression associated with viral infection and identify genes responding differentially to the two viruses. We sampled fire ant queens in Gainesville, Florida, immediately after a mating flight. We arranged them in pairs based on having similar weights (range ±0.2 mg) to allow pleometrotic colony founding. We housed paired queens in nesting tubes in claustral conditions (no food or water) for one month. Thereafter, we collected queens in dry ice, we isolated total RNA from whole bodies and used it to quantify SINV-1 and SINV-2 viruses with real-time PCR. We selected 2 queens that were virus-free as controls, 3 SINV-1-infected, 4 SINV-2-infected and 11 co-infected by both viruses. These samples were processed for microarrays analysis.
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| privacy: |
not applicable
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| aggregation: |
instance of dataset
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| ID: |
E-MTAB-3581
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| refinement: |
raw
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| dateSubmitted: |
12-12-2012
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| keywords: |
functional genomics
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| dateModified: |
01-04-2016
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| creators: |
Fabio Manfredini
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| availability: |
available
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| types: |
gene expression
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| name: |
Solenopsis invicta
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| ID: |
A-MEXP-2297
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| name: |
GrozingerLab S.invicta 12-plex-135k pleo1
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| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3581/E-MTAB-3581.raw.1.zip |
| storedIn: |
ArrayExpress
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| qualifier: |
gzip compressed
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| format: |
TXT
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| accessType: |
download
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| authentication: |
none
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| authorization: |
none
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| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3581/E-MTAB-3581.processed.1.zip |
| storedIn: |
ArrayExpress
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| qualifier: |
gzip compressed
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| format: |
TXT
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| accessType: |
download
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| authentication: |
none
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| authorization: |
none
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| abbreviation: |
EBI
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| homePage: | http://www.ebi.ac.uk/ |
| ID: |
SCR:004727
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| name: |
European Bioinformatics Institute
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| homePage: | https://www.ebi.ac.uk/arrayexpress/ |
| ID: |
SCR:002964
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| name: |
ArrayExpress
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