Title: | Methylation profiling by high throughput sequencing shows that genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking
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dateReleased: |
08-18-2016
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description: |
To address the issue of retention of somatic cell memory in iPSCs we compared methylation profiles of genetically matched human iPSCs derived from fibroblasts and blood. We were using the all-in-one type footprint free SeVdp-iPS system, for generation of uniform iPSC lines. Our results show that iPSC lines derived from the same donor are highly similar to each other. Experimental design: methylation profiles were assayed by Reduced Representation Bisulfite Sequencing (RRBS) for fibroblast-derived iPSC (N=8) and blood-derived iPSC (N=10) as well as isogenic parental fibroblasts and peripheral blood mononuclear cells (N=4 different donors: T14; T42; T53; T55). H9 human embryonic stem cell lines was used as control.
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privacy: |
not applicable
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aggregation: |
instance of dataset
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ID: |
E-MTAB-3859
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refinement: |
raw
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dateSubmitted: |
08-18-2015
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keywords: |
functional genomics
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dateModified: |
09-21-2015
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creators: |
Cristina Valensisi
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availability: |
available
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types: |
gene expression
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name: |
Homo sapiens
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accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3859/E-MTAB-3859.raw.1.zip![]() |
storedIn: |
ArrayExpress
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qualifier: |
gzip compressed
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format: |
TXT
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accessType: |
download
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authentication: |
none
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authorization: |
none
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accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3859/E-MTAB-3859.processed.1.zip![]() |
storedIn: |
ArrayExpress
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qualifier: |
gzip compressed
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format: |
TXT
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accessType: |
download
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authentication: |
none
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authorization: |
none
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abbreviation: |
EBI
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homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
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name: |
European Bioinformatics Institute
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homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
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name: |
ArrayExpress
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