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Title: Methylation profiling by high throughput sequencing shows that genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking      
dateReleased:
08-18-2016
description:
To address the issue of retention of somatic cell memory in iPSCs we compared methylation profiles of genetically matched human iPSCs derived from fibroblasts and blood. We were using the all-in-one type footprint free SeVdp-iPS system, for generation of uniform iPSC lines. Our results show that iPSC lines derived from the same donor are highly similar to each other. Experimental design: methylation profiles were assayed by Reduced Representation Bisulfite Sequencing (RRBS) for fibroblast-derived iPSC (N=8) and blood-derived iPSC (N=10) as well as isogenic parental fibroblasts and peripheral blood mononuclear cells (N=4 different donors: T14; T42; T53; T55). H9 human embryonic stem cell lines was used as control.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3859
refinement:
raw
dateSubmitted:
08-18-2015
keywords:
functional genomics
dateModified:
09-21-2015
creators:
Cristina Valensisi
availability:
available
types:
gene expression
name:
Homo sapiens
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3859/E-MTAB-3859.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3859/E-MTAB-3859.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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