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Title: Gpx3 male transgenic liver expression vs B6/DBA male wildtype control      
dateReleased:
03-07-2009
description:
A major task in dissecting the genetics of complex traits is to identify causal genes for disease phenotypes. We previously developed a method to infer causal relationships among genes through the integration of DNA variation, gene transcription, and phenotypic information. Here we validated our method through the characterization of transgenic and knockout mouse models of candidate genes that were predicted to be causal for abdominal obesity. Perturbation of eight out of the nine genes, with Gas7, Me1 and Gpx3 being novel, resulted in significant changes in obesity related traits. Liver expression signatures revealed alterations in common metabolic pathways and networks contributing to abdominal obesity and overlapped with a macrophage-enriched metabolic network module that is highly associated with metabolic traits in mice and humans. Integration of gene expression in the design and analysis of traditional F2 intercross studies allows high confidence prediction of causal genes, and identification of involved pathways and networks. Keywords: Obesity study in transgenic animals A total of 8 mice were used for this study (4 Gpx3 transgenic and 4 B6/DBA wildtype). The gene expression data is from the livers of 23 week old male wildtype and transgenic mice. These mice were fed a 4% fat chow diet until 11 weeks of age and then were fed a 6% fat chow diet until sacrifice at 23 weeks of age.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-11997
refinement:
raw
alternateIdentifiers:
11997
dateSubmitted:
07-02-2008
keywords:
functional genomics
dateModified:
06-10-2011
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-4865
name:
Sentrix MouseRef-8 Expression BeadChip (Target ID)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-11997/E-GEOD-11997.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-11997/E-GEOD-11997.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11997
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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