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Title: Transcriptional profiling by array of Entamoeba histolytica Mutant Derived from Virulent Strain      
dateReleased:
05-15-2015
description:
Amoebiasis is a disease caused by the protozoan parasite, Entamoeba histolytica, with or without clinical symptoms . It has been suggested that pathogenic amoebae use three major virulence factors: Gal/GalNAc lectin, cysteine proteinases and amoebapores. However, these molecules cannot exclusively be responsible for amoebic virulence, because most of them are found in pathogenic E. histolytica as well as in non-pathogenic E. dispar, a not close correlation has been found in amoebic strains with different virulence phenotype. Due to the high genetic variability of E. histolytica isolates and strains, differential gene expression might simply reflect inter-isolate genetic variation rather than specific differences linked to virulence. In the current study, we obtained a non-virulent UG10 mutant derived from E. histolytica HM-1:IMSS. Comparing the transcriptome of both strains, no differences in gene expression of the classical virulence factors were observed. RAB family GTPase and AIG1 family members showed higher transcriptional levels in the virulent strain than in the non-virulent mutant. Our study suggests a number of novel gene products in E. histolytica with a role yet to determine in cell physiology and involvement in the pathogenesis process.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3525
refinement:
raw
dateSubmitted:
05-25-2010
keywords:
functional genomics
dateModified:
04-23-2015
creators:
Bernardo Franco Barcenas
availability:
available
types:
gene expression
name:
Entamoeba histolytica
name:
cell type comparison design
ID:
A-GEOD-4622
name:
Entamoeba histolytica E_his-1a520285F array (coding regions)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3525/E-MTAB-3525.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3525/E-MTAB-3525.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress