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Title: Expression data for human nasopharyngeal carcinoma cell line HK1 stimulated with EGF or TGF-β recombinant protein      
dateReleased:
12-17-2015
description:
As regulators in gene expression, microRNAs take part in most biological process including cell differentiation, apoptosis, cell cycle and epithelial-to-mesenchymal transition (EMT). In order to evaluate their roles during the growth factors-induced EMT process, microRNA expression profile changes induced by EGF or TGF-β treatment on nasopharyngeal carcinoma cell line HK-1 cells were analyzed by means of the Affymetrix genome wide microarray system. Human nasopharyngeal carcinoma cell line HK-1 was starved for 12 hours before stimulation with EGF (50 ng/mL) or TGF-β (10ng/ml) or Control (PBS) for 36 hours in RPMI-1640 medium (with 1% serum) to establish the in vitro model of EMT. Total RNA were extracted and detected by Affymetrix® GeneChip® miRNA Arrays.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-64281
refinement:
raw
alternateIdentifiers:
64281
keywords:
functional genomics
dateModified:
12-18-2015
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-14613
name:
[miRNA-2_0] Affymetrix Multispecies miRNA-2_0 Array
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-64281/E-GEOD-64281.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-64281/E-GEOD-64281.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64281
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress