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Title: MDM33 overexpression suppressor screen      
dateReleased:
01-12-2016
description:
We performed a genome-wide screen for genetic MDM33 interaction partners. Using the opposite approach of the well known synthetic dosage lethality (screening for deletion strains in which a non-toxic overexpression is associated with a synthetic growth arrest) we screened the whole genome for genes that are essential for the growth arrest associated with MDM33 overexpression. In a pooled collection of viable haploid gene deletion mutants, of which each is tagged with a unique identifying molecular ‘‘bar-code’’ sequence, the overexpression of MDM33 was induced using a galactose responsive promotor. While overexpression resulted in a growth arrest in almost all strains a small fraction of deletion mutants was able to grow under inductive conditions. We identified these strains using a microarray DNA hybridization technique that quantifies the abundance of the molecular barcodes. We propose that the affected genes are possible functional genetic interaction partners of MDM33. Replicate set 1: knockout strains transformed with empty vector and MDM33 overexpression vector; each transformation plated on glucose and galactose media; uptags and downtags were labelled and detected independently; Replicate set 2: same as replicate set 1, plus dye-swap;
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-49580
refinement:
raw
alternateIdentifiers:
49580
keywords:
functional genomics
dateModified:
01-16-2016
availability:
available
types:
gene expression
name:
Saccharomyces cerevisiae
ID:
A-GEOD-17535
name:
Agilent-025581 UBT-ZBv1
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-49580/E-GEOD-49580.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-49580/E-GEOD-49580.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE49580
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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