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Title: Molecular classification of multiple myeloma  
description:
PURPOSE. The deregulation of CCND1, CCND2 and CCND3 genes represents a common event in multiple myeloma (MM). The recently proposed TC classification grouped MM patients into five classes on the basis of their cyclins D expression profiles and the presence of the main translocations involving the immunoglobulin heavy-chain locus (IGH) at 14q32. In this study, we provide a molecular characterization of the identified TC groups. MATERIALS AND METHODS. The gene expression profiles of purified plasma cells from 50 MM cases were used to stratify the samples into the five TC classes and identify their transcriptional fingerprints. The cyclin D expression data were validated by means of real-time quantitative PCR analysis; fluorescence in-situ hybridization was used to investigate the cyclin D loci arrangements, and to detect the main IGH translocations and the chromosome 13q deletion. RESULTS. Class-prediction analysis identified 112 probe sets as characterizing the TC1, TC2, TC4 and TC5 groups, whereas the TC3 samples showed heterogeneous phenotypes and no marker genes. The TC2 group, which showed extra copies of the CCND1 locus and no IGH translocations or the chromosome 13q deletion, was characterized by the overexpression of genes involved in protein biosynthesis at translational level. A meta-analysis of published datasets validated the identified gene expression signatures. CONCLUSIONS. Our data contribute to the understanding of the molecular and biological features of distinct MM subtypes. The identification of a distinctive gene expression pattern in TC2 patients may improve risk stratification and indicate novel therapeutic targets. Last Updated (by provider): Dec 15 2010 Contributers: Vincenzo Callea Luigia Lombardi Daniela Intini Luca Agnelli Antonino Neri Michela Mattioli Donata Verdelli Luca Baldini Sonia Fabris Silvio Bicciato Fortunato Morabito Includes GDS1284. Update date: Oct 05 2006. Dataset description GDS1284: Analysis of CD138+ plasma cells purified from bone marrow of multiple myeloma (MM) patients. Results used to classify MM cases into translocation/cyclin (TC) groups based on cyclin D expression and presence of translocations in the immunoglobulin heavy chain locus at 14q32..
privacy:
not applicable
aggregation:
instance of dataset
ID:
2130
refinement:
curated
keywords:
functional genomics
availability:
available
types:
gene expression
ID:
GEO:GSE2912
accessURL: http://www.chibi.ubc.ca/Gemma/expressionExperiment/showExpressionExperiment.html?id=2130
storedIn:
Gemma
qualifier:
filtered
format:
TSV
accessType:
Submit Job
authentication:
none
authorization:
none
accessURL: http://www.chibi.ubc.ca/Gemma/expressionExperiment/showExpressionExperiment.html?id=2130
storedIn:
Gemma
qualifier:
unfiltered
format:
TSV
accessType:
Submit Job
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE2912
storedIn:
Gene Expression Omnibus
format:
HTML
accessType:
Landing Page
primary:
true
authentication:
none
authorization:
none
abbreviation:
UBC
homePage: http://www.ubc.ca/
ID:
SCR:011614
name:
University of British Columbia
homePage: http://www.chibi.ubc.ca/Gemma
ID:
SCR:008007
name:
Gemma