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Title: Comparison of Environmental and Genetic models of ADHD  
description:
ADHD is the most common neurobehavioral disorder in school-aged children. In addition to genetic factors, environmental influences or gene x environmental interactions also play an important role in ADHD. One example of a well studied environmental risk factor for ADHD is exposure to polychlorinated biphenyls (PCBs). In this study, we investigated whether the well-established genetic model of ADHD based on the Spontaneously Hypertensive Rat (SHR) and a well established PCB-based model of ADHD exhibited similar molecular changes in brain circuits involved in ADHD. The brains from 28 male rats (8 SHR, 8 Sprague-Dawley (SD) controls, 8 Wistar-Kyoto (WKY) controls, and 4 PCB-exposed SD rats) were harvested at postnatal day 55-65 and RNA was isolated from six brain regions of interest. The RNA was analyzed for differences in expression of a set of 308 probe sets interrogating 218 unique genes considered highly relevant to ADHD or epigenetic gene regulation using the Rat RAE 230 2.0 GeneChip (Affymetrix). Selected observations were confirmed by real time quantitative RT-PCR. The results show that the expression levels of genes Gnal, COMT, Adrbk1, Ntrk2, Hk1, Syt11 and Csnk1a1 were altered in both the SHR rats and the PCB-exposed SD rats. Arrb2, Stx12, Aqp6, Syt1, Ddc and Pgk1 expression levels were changed only in the PCB-exposed SD rats. Genes with altered expression only in the SHRs included Oprm1, Calcyon, Calmodulin, Lhx1 and Hes6.The epigenetic genes Crebbp, Mecp2 and Hdac5 are significantly altered in both models. The data provide strong evidence that genes and environment can affect different set of genes in two different models of ADHD and yet result in the similar disease-like symptoms. Last Updated (by provider): Mar 15 2009 Contributers: Stephen V Faraone Terje Sagvolden David F Berger Frank A Middleton John P Lombardo Tania DasBanerjee
privacy:
not applicable
aggregation:
instance of dataset
ID:
1168
refinement:
curated
keywords:
functional genomics
availability:
available
types:
gene expression
ID:
GEO:GSE12457
accessURL: http://www.chibi.ubc.ca/Gemma/expressionExperiment/showExpressionExperiment.html?id=1168
storedIn:
Gemma
qualifier:
filtered
format:
TSV
accessType:
Submit Job
authentication:
none
authorization:
none
accessURL: http://www.chibi.ubc.ca/Gemma/expressionExperiment/showExpressionExperiment.html?id=1168
storedIn:
Gemma
qualifier:
unfiltered
format:
TSV
accessType:
Submit Job
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12457
storedIn:
Gene Expression Omnibus
format:
HTML
accessType:
Landing Page
primary:
true
authentication:
none
authorization:
none
abbreviation:
UBC
homePage: http://www.ubc.ca/
ID:
SCR:011614
name:
University of British Columbia
homePage: http://www.chibi.ubc.ca/Gemma
ID:
SCR:008007
name:
Gemma

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