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Title: Neutralizing Antibodies against West Nile Virus from Human B Cells      
citations:
26481611
description:
West Nile virus (WNV) infection is a mosquito-borne disease that can cause severe neurological illness. We analyzed the humoral response to WNV of subjects infected with WNV at different stages of the infection and at different levels of detail, from single cells to the repertoire level. We integrated single-cell analysis by microengraving with next-generation sequencing and identified four novel WNV neutralizing antibodies with potential use as therapeutics against WNV infection. The results indicate persistence of WNV-specific memory B cells and antibody-secreting cells in post-convalescent subjects and suggest that the antibody response itself does not predict the clinical severity of the disease.
Identifier:
phs001200.v1.p1
accesstypes:
download
enclave
landingpage: http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001200.v1.p1
authentication: simpleLogin
none
authorization:
none
duaIndividual
name:
West Nile Fever
West Nile virus
affiliations:
Yale University, New Haven, CT, USA
name:
Ruth R Montgomery, PhD
roles:
Principal Investigators
affiliations:
Yale University, New Haven, CT, USA
name:
Steven H Kleinstein, PhD
roles:
Principal Investigators
affiliations:
Massachusetts Institute of Technology, Cambridge, MA, USA
name:
J. Christopher Love, PhD
roles:
Principal Investigators
affiliations:
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
name:
U19AI089992
roles:
Funding Source
affiliations:
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
name:
U19AI089859
roles:
Funding Source
performedBy:
TitleNameInstitute Principal InvestigatorsRuth R Montgomery, PhDYale University, New Haven, CT, USA Principal InvestigatorsSteven H Kleinstein, PhDYale University, New Haven, CT, USA Principal InvestigatorsJ. Christopher Love, PhDMassachusetts Institute of Technology, Cambridge, MA, USA Funding SourceU19AI089992National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA Funding SourceU19AI089859National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
downloadURL: https://dbgap.ncbi.nlm.nih.gov/aa/wga.cgi?page=DUC&view_pdf&stacc=phs001200.v1.p1
Identifier:
phs001200.v1.p1_policy
name:
Data Use Certificate
Identifier:
3
name:
Disease-Specific (Immunological Research, IRB)
alternateIdentifiers:
yes
selectionCriteria:

Confirmation of West Nile Virus infection
Diagnosis and stratification of WNV infection were determined following CDC guidelines and definitions to a severe neuroinvasive phenotype, mild fever-only phenotype, or asymptomatic infection. Infection was validated by qualitative rapid nucleic acid test at the blood bank, positive immunoblot or IgM ELISAs.

types:
Case Set
dateReleased:
08-26-2016
version:
phs001200.v1.p1
dateModified:
02-21-2017
abbreviation:
NIAID
name:
NIAID DAC
ID:
0
name:
dbGaP

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