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Metadata

Name
ATCC Even Mock Community full length 16S rRNA gene reference sequences in DADA2 format
Repository
ZENODO
Identifier
doi:10.5281/zenodo.4781067
Description
After an informative Twitter conversation about 16S sequencing bias and the importance of mock communities. I decided to&nbsp;share some of the files I had that might also help others do good microbiome analysis. The files contain&nbsp;multiple 16S rRNA sequences belonging to the 20 species reported in the ATCC even mock community and other fasta.

The ones you need for mock analysis are only the following&nbsp;two fasta files formatted for use in dada2 assignTaxonomy command (ATCC_Even_Mock_community_GTDBr202.fa&nbsp;&amp; ATCC_Even_Mock_community_RefSeq.fa). to create them I used the most recent RefSeq +RDP database on my other zenodo page (included here too) and the entire GTDB ssu collection after formatting the fasta headers (&nbsp;uploaded it here too). I have also included information I have found about the ATCC mock species and created a key to find the GTDB taxonomy.

The use case as I see it is that you can query these databases with your samples if you are using the ATCC mock community as a positive/bias control in your microbiome experiment. You can probably also use it with this awesome work by Sudarshan Shetty here https://microsud.github.io/chkMocks/articles/cExampleCustomMocks.html .

You can also do as you please with it :-). If you need me to assist or anything microbiome my email is&nbsp;alishum.ali@postgrad.curtin.edu.au

Everything I used is uploaded, happy days.&nbsp;

The primitive bash code is to help you scientists reproduce the fasta&nbsp;files.
Data or Study Types
multiple
Source Organization
Unknown
Access Conditions
available
Year
2021
Access Hyperlink
https://doi.org/10.5281/zenodo.4781067

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