Metadata
- Name
- Single-cell RNA sequencing data from hiPSC disease modeling of Loeys-Dietz Syndrome (LDS)
- Repository
- Gene Expression Omnibus
- Identifier
- geo.series:GSE175647
- Description
- For in vitro disease modeling of LDS, we used healthy donor cells (TGFBR1+/+), and generated heterozygote (TGFBR1A230T/+), and homozygote (TGFBR1A230T/A230T) knock-in clones using CRISPR-Cas9 gene editing. We also generated hiPSC from peripheral blood mononuclear cells harvested from an LDS patient, and corrected the mutation in the patient-derived hiPSC. To address the lineage-specific effects of TGFBR1A230T, and SMAD3c.652delA/+, hiPSC were differentiated into cardiovascular progenitor cell-derived smooth muscle cells (CPC-SMC), and neural crest stem cell-derived smooth muscle cells (NCSC-SMC) using in vitro differentiation protocols. We performed large-scale single cell profiling of the resulting CPC-SMC and NCSC-SMC.
- Data or Study Types
- Expression profiling by high throughput sequencing
- Source Organization
- National Center for Biotechnology Information
- Access Conditions
- available
- Year
- 2021
- Access Hyperlink
- http://www.ncbi.nlm.nih.gov/sites/GDSbrowser?acc=GSE175647
Distributions
- Encoding Format: Bioproject ; URL: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA733141
- Encoding Format: TXT ; URL: ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE1nnn/GSE175647/matrix/
- Encoding Format: MINiML ; URL: ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE1nnn/GSE175647/miniml/
- Encoding Format: SOFT ; URL: ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE1nnn/GSE175647/soft/