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Metadata

Name
Microbiome diversity and reproductive incompatibility induced by the prevalent endosymbiont Arsenophonus in two species of African cassava Bemisia tabaci whiteflies
Repository
ZENODO
Identifier
doi:10.5061/dryad.xsj3tx9gc
Description
This dataset contains data from two-part experiments described in the paper: "El Hamss, H., Ghosh, S., M. N., M., Delatte, H., &amp; Colvin, J. (2021). Microbiome diversity and reproductive incompatibility induced by the prevalent endosymbiont Arsenophonus in two species of African cassava Bemisia tabaci whiteflies. Ecology and Evolution, 00, 1–10. https://doi.org/10.1002/ece3.840".

The experiment investigates the effects of Arsenophonus on whitefly reproduction and microbiome diversity.

In the first experiment ("crossing experiment"), the effect of Arsenophonus is studied on number of eggs, nymphs, males, and females on different whitefly species at intraspecies level (SSA1-SG3A+ with SSA1-SG3A-) and at interspecies level (SSA2A+ with SSA1-SG3A- and SSA1-SG3A+). Arsenophonus infection A+ means the presence of Arsenophonus in related whitefly colony, A- means the absence of Arsenophonus.

In the second experiment ("microbiome diversity"), whitefly colonies of crossed parents and generated progenies were sequenced to check Arsenophonus threshold (number of reads) and the presence of other bacteria in those crossed whiteflies.

The main results of this work are:

(i) Arsenophonus did not induce reproductive incompatibility within SSA1-SG3 but reduced the number of eggs, nymphs and female ratios,

(ii) complete RI was observed between SSA1-SG3 and SSA2 indicating the lack of gene flow between the two whitefly species,

(iii) many new 'other bacteria' in SSA B. tabaci have been identified, whose role remains to be investigated.
Data or Study Types
multiple
Source Organization
Unknown
Access Conditions
available
Year
2021
Access Hyperlink
https://doi.org/10.5061/dryad.xsj3tx9gc

Distributions

  • Encoding Format: HTML ; URL: https://doi.org/10.5061/dryad.xsj3tx9gc
whiteflymicrobiomediversityspeciestwotabaciafricancassavaeggscontainsdatadescribedamporgiiirole
This project was funded in part by grant U24AI117966 from the NIH National Institute of Allergy and Infectious Diseases as part of the Big Data to Knowledge program. We thank all members of the bioCADDIE community for their valuable input on the overall project.