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Title: Transcription profiling by array of E. coli K12 IHF and HU single and double deletion mutants      
availability:
available
aggregation:
instance of dataset
privacy:
not applicable
refinement:
curated
dateReleased:
12-21-2011
ID:
E-MEXP-3461
description:
IHF and HU are two heterodimeric nucleoid associated proteins (NAP) that belong to the same protein family but interact differently with the DNA. IHF is a sequence-specific DNA-binding protein that bends the DNA by over 160o. HU is the most conserved NAP which binds non-specifically to duplex DNA with a particular preference for targeting nicked and bent DNA. Despite their importance, the in-vivo interactions of the two proteins to the DNA remain to be described at a high resolution and on a genome-wide scale. Further, the effects of these proteins on gene expression on a global scale remain contentious. Finally, the contrast between the functions of the homo- and heterodimeric forms of these proteins deserves the attention of further study. Here we present a genome-scale study of HU- and IHF-binding to the E. coli K12 chromosome using ChIP-seq. We also perform microarray analysis of gene expression in single- and double-deletion mutants of each protein to identify their regulons. This data is for microarrays measuring gene expression changes in the various ihf mutants when compared to the wildtype.
keywords:
transcription profiling by array
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HTML
storedIn:
Array Express
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not compressed
accessType:
landing page
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none
authentication:
none
primary:
true
accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-3461
format:
JSON
storedIn:
OmicsDI
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not compressed
accessType:
download
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none
authentication:
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primary:
false
accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-MEXP-3461.json
format:
XML
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-MEXP-3461.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/