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Title: Comparison of B. japonicum 110spc4 (wild type) induced versus uninduced      
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available
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not applicable
refinement:
curated
dateReleased:
03-27-2012
ID:
E-GEOD-8571
description:
An initializing step in the rhizobia-legume symbiosis is the secretion of flavonoids by plants that leads to the expression of nodulation genes in rhizobia. Here we report the genome-wide transcriptional response of Bradyrhizobium japonicum to genistein, an isoflavone secreted by soybean. About 100 genes were induced in the wild type. This included all nod box-associated genes, the flagellar cluster and several genes that are likely to be involved in transport processes. To elucidate the role of known regulators, we analysed mutant strains. This revealed that the two-component response regulator NodW is essential for induction of almost all genistein-inducible genes, with the exception of 8 genes. The phenotype of the nodW mutant could be partially suppressed by overexpression of NwsB, which is also a two-component response regulator. These data indicate that genistein has a much broader function than mere induction of nod genes. Keywords: transcriptional analysis, genistein induction 3 biological replicates of wild type uninduced and wild type induced with genistein were compared.
keywords:
transcription profiling by array
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storedIn:
Array Express
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true
accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8571
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OmicsDI
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accessType:
download
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accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-8571.json
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XML
storedIn:
OmicsDI
qualifier:
not compressed
accessType:
download
authorization:
none
authentication:
none
primary:
false
accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-8571.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/

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