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Title: Polysome-associated mRNA levels upon glucose repletion      
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available
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instance of dataset
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not applicable
refinement:
curated
dateReleased:
05-02-2014
ID:
E-GEOD-31220
description:
Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy following glucose withdrawal show that most mRNAs are lost from the cell coincident with their loss from polysomes. Second, only a very limited sub-population of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose re-addition in the absence of new transcription. This highly selective post-transcriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth. Sigma 1278b yeast were grown to OD600=1.0-1.1 in YPD in baffled flasks at 30C with vigorous shaking. Cells were treated with thiolutin to a final concentration of 3ug/mL, harvested by centrifugation, and resuspended in pre-warmed YPA medium lacking glucose. Cells were returned to 30C for shaking for 10 minutes. For the refed samples, glucose was added to a final concentration of 2% and samples were taken after 5 minutes. Polysome preparation and RNA isolation were as described in Experimental Procedues. A common RNA sample, polysomal RNA from cells grown in YPD, was used for one channel of each array.
keywords:
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transcription profiling by array
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storedIn:
Array Express
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accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-31220
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JSON
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OmicsDI
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download
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accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-31220.json
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OmicsDI
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accessType:
download
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accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-31220.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/