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Title: Ruler Arrays Reveal Haploid Genomic Structural Variation      
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available
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instance of dataset
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not applicable
refinement:
curated
dateReleased:
05-02-2014
ID:
E-GEOD-23524
description:
A ruler array combines novel sample preparation protocols, tiling genomic microarrays, and a new computational method to turn each probe on a microarray into a ruler that measures the physical distance between defined genomic sequences regardless of the intervening sequence. The ruler array protocol involves (1) genomic DNA purification (2) digestion with a restriction enzyme (3) ligation of a biotinylated adapter molecule to the cut sites (4) purification on streptavidin beads (5) polymerase extensions from a primer complementary to the adapter using labeled dNTPs and (6) hybridization to a tiling microarray Two replicates of our ruler array experiment to detect insertions and deletions between two genomic samples of strains FY4 and Sigma1278b.
keywords:
comparative genomic hybridization by array
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Array Express
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accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23524
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OmicsDI
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accessType:
download
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accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-23524.json
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XML
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OmicsDI
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accessType:
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accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-23524.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/