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Title: Transcription profiling by array of Arabidopsis mutant for sdh1 after treatment with salicylic acid      
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available
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instance of dataset
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not applicable
refinement:
curated
dateReleased:
04-30-2015
ID:
E-GEOD-22942
description:
Reactive oxygen species such as hydrogen peroxide (H2O2) are important in biotic and abiotic stress responses in plants, but their source is often unclear. We have identified an Arabidopsis mutant that shows loss of stress responsive GSTF8 gene expression in response to the plant defence signal salicylic acid (SA) . The mutant showed increased susceptibility to both fungal and bacterial pathogens. The dsr1 mutation was mapped to mitochondrial succinate dehydrogenase (SDH1-1) and dsr1 had reduced SDH activity and a lowered mitochondrial H2O2 production. To better understand the loss of SA-response in dsr1, we performed ATH1 microarray analysis in WT and dsr1 10 hours after a 1 mM SA treatment, a point at which we observed maximal SA-induced GSTF8 promoter activity. 4 day old WT (a Columbia line containing a -800GSTF8::LUC reporter) and dsr1 seedlings (a mutant Columbia line containing a -800GSTF8::LUC) reporterwere treated with water (mock) or 1 mM SA for 40 min. Ten hours after treatment, samples were collected.
keywords:
transcription profiling by array
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storedIn:
Array Express
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true
accessURL: https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22942
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OmicsDI
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accessType:
download
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accessURL: www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-22942.json
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XML
storedIn:
OmicsDI
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not compressed
accessType:
download
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none
authentication:
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primary:
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accessURL: http://www.omicsdi.org/ws/dataset/arrayexpress-repository/E-GEOD-22942.xml
ID:
SCR:014747
name:
Omics Discovery Index
abbreviation:
OmicsDI
homePage: http://www.omicsdi.org/