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Title: Molecular Architecture Of The Yeast Mediator Complex      
dateReleased:
10-13-2016
privacy:
information not avaiable
aggregation:
instance of dataset
dateCreated:
10-13-2016
refinement:
raw
ID:
doi:10.5281/ZENODO.160593
creators:
Robinson, Philip J
Trnka, Michael J
Pellarin, Riccardo
Greenberg, Charles H
Bushnell, David A
Davis, Ralph
Burlingame, Alma L
Sali, Andrej
Kornberg, Roger D
availability:
available
types:
other
description:
The 21-subunit Mediator complex transduces regulatory information from enhancers to promoters, and performs an essential role in the initiation of transcription in all eukaryotes. This repository contains files used in the 3-D modeling of the entire Mediator complex, using an integrative modeling approach that combines information from chemical cross-linking and mass spectrometry; X-ray crystallography; homology modeling; and cryo-electron microscopy. The approach is validated by the use of X-ray crystal structures as internal controls and by consistency with previous results from electron microscopy and yeast two-hybrid screens. The model shows the locations and orientations of all Mediator subunits, as well as subunit interfaces and some secondary structural elements. Segments of 20–40 amino acid residues are placed with an average precision of 20 Å. The model reveals roles of individual subunits in the organization of the complex.
accessURL: https://doi.org/10.5281/ZENODO.160593
storedIn:
Zenodo
qualifier:
not compressed
format:
HTML
accessType:
landing page
authentication:
none
authorization:
none
abbreviation:
ZENODO
homePage: https://zenodo.org/
ID:
SCR:004129
name:
ZENODO