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Title: Analysis Of The Lac Operon Induction With Single Cell Resolution Using The Dimm Microfluidics Chip And The Moma Software      
dateReleased:
01-23-2017
privacy:
information not avaiable
aggregation:
instance of dataset
dateCreated:
01-23-2017
refinement:
raw
ID:
doi:10.5281/ZENODO.257566
creators:
Kaiser, Matthias
Jug, Florian
Julou, Thomas
Deshpande, Siddharth
Pfohl, Thomas
Silander, Olin
Myers, Gene
Van Nimwegen, Erik
availability:
available
types:
other
description:
In this work, we demonstrate the power of the DIMM (Dual Input Mother Machine), a new microfluidics setup allowing long term environmental control and single cell resolution, and MoMA (Mother Machine Analyzer), its companion image analysis sofware. We study the induction of the lac operon in Escherichia coli MG1655 when nutrients alternate between glucose and lactose, using a translational fusion of LacZ with GFP integrated at the endogeneous locus. The dataset is made of two archives: DIMM_MoMA_images.tar.gz contains the image data produced in this study in a form ready to be used in MoMA DIMM_MoMA_data.tar.gz contains textual data files produced by MoMA and its postprocessing, with the cell identifier, length and fluorescence intensity of each cell in each frame. Detailed instructions are provided in the ReadMe.md file (NB: this is a text file using markdown syntax, you can open it in your favorite text editor).
accessURL: https://doi.org/10.5281/ZENODO.257566
storedIn:
Zenodo
qualifier:
not compressed
format:
HTML
accessType:
landing page
authentication:
none
authorization:
none
abbreviation:
ZENODO
homePage: https://zenodo.org/
ID:
SCR:004129
name:
ZENODO

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