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Title: The genome of the Australian dragon lizard Pogona vitticeps      
dateReleased:
09-22-2015
privacy:
not applicable
aggregation:
instance of dataset
dateCreated:
09-22-2015
refinement:
curated
ID:
doi:10.5524/100166
creators:
Georges, Arthur
,
OMeally, Denis
Deakin, Janine
Zhang, Pei
Fujita, Matthew
Patel, Hardip R
Holleley, Clare E
Zhou, Yang
Matsubara, Kazumi
Waters, Paul
Graves, Jennifer AM
Sarre, Stephen D
availability:
available
types:
sequence
description:
We present a genomic resource for a wild-caught male ZZ Central Bearded Dragon Pogona vitticeps, from Australia. The genomic sequence, generated on the Illumina HiSeq 2000 platform, comprised 317 Gbp (185x raw read depth) from 13 insert libraries ranging from 250 bp to 40 Kbp. After filtering for low quality and duplicated reads, a total of 146 Gbp of data (86x) was available for assembly. Exceptionally high levels of heterozygosity (0.85 % SNP plus indels) complicated assembly, nevertheless 96.4% of reads mapped back to the assembled scaffolds indicating that the assembly included most of the sequenced genome. The total length of the assembly was 1.8 Gbp in 545,310 scaffolds (69,852 longer than 300 bp), the longest being 14.68 Mbp, with an N50 of 2.29 Mbp. We also present transcriptome data from brain, heart, lung, liver, kidney, skeletal muscle and gonads of male and female P. vitticeps, assembled these datasets (from seven individuals) into 595,564 contigs. Genes were annotated on the basis of de novo prediction, similarity to Anolis carolinensis, Gallus gallus and Homo sapiens proteins, and Pogona vitticeps transcriptome sequence assemblies, to yield 19,406 protein-coding genes in the assembly, 63% of which had intact open reading frames. Our assembly captured 99% (246 of 248) of the core CEGMA genes, with 93% (231) being complete. The annotated Pogona genome can be accessed through a genome browser available at https://genomics.canberra.edu.au.
accessURL: https://doi.org/10.5524/100166
storedIn:
GigaScience Database
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
GigaDB
homePage: http://gigadb.org/
ID:
SCR:006565
name:
Giga Science Database