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Title: Supporting data for "A reference gene set construction using RNA-seq of multiple tissues of Chinese Giant Salamander, Andrias davidianus"      
dateReleased:
02-10-2017
privacy:
not applicable
aggregation:
instance of dataset
dateCreated:
02-10-2017
refinement:
curated
ID:
doi:10.5524/100277
creators:
,
Gou, Qiang
Li, Jiang
Wang, Ying
Ma, Ji
Chen, Bing
Wei, Hong
availability:
available
types:
sequence
description:
Chinese giant salamander (CGS) is the largest extant amphibian species in the world. Owing to its evolutionary position and four peculiar phenomenon of life (longevity, starvation tolerance, regenerative ability, and hatch without sunshine), it is an invaluable model species for research. However, lack of genomic resources and its huge (~50 GB) difficult to assemble genome, leads to slow study progression in this field. Here we report the sequenced transcriptome of more than twenty tissues from adult specimens using Illumina Hiseq 2000 technology, and a total of 93,366 non-redundant transcripts with a mean length of 1,326 bp. We for the first time developed an efficient pipeline to construct a high quality reference gene set of CGS and obtained 26,135 coding genes. BUSCO and homologous assessment showed that our assembly captured 70.6 % of vertebrate universal single-copy orthologs.
accessURL: https://doi.org/10.5524/100277
storedIn:
GigaScience Database
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
GigaDB
homePage: http://gigadb.org/
ID:
SCR:006565
name:
Giga Science Database

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