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Title: CAS9_STRP1      
description:
CRISPR-associated endonuclease Cas9/Csn1 HNH Cas9-type RuvC-I Recognition lobe ARM RuvC-II RuvC-III PAM-interacting domain (PI) PAM substrate-binding For RuvC-like nuclease domain Proton acceptor for HNH nuclease domain Manganese 1 Manganese 2 Manganese 1 Manganese 1 Manganese 2 Manganese 2; via pros nitrogen Manganese 3; via tele nitrogen Manganese 3 Target DNA noncomplementary to the crRNA is not cleaved; nickase activity. Processes guide RNAs. In vivo, loss of Cas9-mediated CRISPR interference in plasmid transformation. Able to bind guide RNAs and target DNA but not cleave DNA; when associated with A-840 Decreased DNA cleavage Significantly decreased DNA cleavage No DNA cleavage Significantly decreased DNA cleavage Moderately decreased DNA cleavage No nuclease activity Moderately decreased DNA cleavage About 50% nuclease activity No nuclease activity Slight decrease in target DNA cleavage and DNA-binding. Almost complete loss of DNA cleavage and binding; when associated with 1125-A--A-1127 Only cleaves 1 DNA strand, probably the noncomplementary strand. Processes guide RNAs correctly. In vivo, loss of Cas9-mediated CRISPR interference in plasmid transformation Target DNA complementary to the crRNA is not cleaved; nickase activity. In vivo, loss of Cas9-mediated CRISPR interference in plasmid transformation. Able to process and bind guide RNAs and target DNA but not cleave DNA; when associated with A-10 Decreased DNA cleavage. Processes guide RNAs correctly. In vivo, retains Cas9-mediated CRISPR interference in plasmid transformation Only cleaves 1 DNA strand, probably the complementary strand. Processes guide RNAs correctly. In vivo, loss of Cas9-mediated CRISPR interference in plasmid transformation Processes guide RNAs correctly Decreased DNA cleavage. In vivo, loss of Cas9-mediated CRISPR interference in plasmid transformation Only cleaves 1 DNA strand, probably the noncomplementary strand Only cleaves 1 DNA strand, probably the noncomplementary strand. Processes guide RNAs correctly. In vivo, loss of Cas9-mediated CRISPR interference in plasmid transformation No nuclease activity No change in target DNA cleavage, slight decrease in DNA-binding. Almost complete loss of DNA cleavage and binding; when associated with 475-A--A-477 Probably inactivates protein Nearly complete loss of target DNA cleavage Dramatically reduced target DNA binding, slightly decreased target cleavage Dramatically reduced target DNA binding, slightly decreased target cleavage
types:
sequence
dateReleased:
06-01-2001
ID:
Q99ZW2
privacy:
not applicable
keywords:
3D-structure
Antiviral defense
Complete proteome
DNA-binding
Endonuclease
Exonuclease
Hydrolase
Magnesium
Manganese
Metal-binding
Nuclease
Reference proteome
RNA-binding
availability:
available
refinement:
curated
version:
1
aggregation:
instance of dataset
name:
S
ID:
ncbitax:301447
name:
CRISPR-associated endonuclease Cas9/Csn1
authorization:
none
authentication:
none
format:
HTML
storedIn:
UniProt
accessType:
landing page
qualifier:
not compressed
accessURL: http://www.uniprot.org/uniprot/Q99ZW2
license:
Creative Commons Attribution-NoDerivs License
authorization:
none
authentication:
none
format:
FASTA
storedIn:
UniProt
accessType:
download
qualifier:
not compressed
accessURL: http://www.uniprot.org/uniprot/Q99ZW2.fasta
license:
Creative Commons Attribution-NoDerivs License
name:
European Bioinformatics Institute
abbreviation:
EMBL-EBI
ID:
SCR:004727
homePage: http://www.ebi.ac.uk/
name:
UniProt
abbreviation:
UniProt
ID:
SCR:002380
homePage: http://www.uniprot.org/

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