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Title: Gene expression data from WT and TET2cKO CD8+ T cells activated in vitro      
keywords:
Transcriptome or Gene expression
ID:
PRJNA376189
description:
The epigenetic modifier TET2 plays a role in cell fate decisions in hematopeotic stem cells and CD4+ Th1 and Th17 differentiation. Here, we demonstrate that loss of TET2 promotes CD8+ memory differentiation following acute viral infection with LCMV-Armstrong. To identify early gene expression changes following TCR activation in WT versus TET2cKO CD8+ T cells, we isolated naive CD8+ T cells and activated them for three days with anti-CD3/CD28+IL-2 and performed microarray analysis. Overall design: Naive (CD44lo CD62Lhi) CD8+ T cells were isolated and sorted from WT C57BL/6 and TET2fl/flCD4Cre+ mice. These cells were activated with plate-bound anti-CD3/CD28 for 3 days in the presence of 100U/ml hIL-2. RNA from these in vitro activated CD8+ T cells were processed, amplified, labeled and hybridized to Affymetrix GeneChip MoGene 2.0 ST microarrays.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA376189
authentication:
none
authorization:
none
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject