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Title: Biroeactor scalability : Bioreactor scalability: laboratory-scale bioreactor design influences bioreactor performance, ecology and community physiology in expanded granular sludge bed bioreactors      
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ID:
PRJEB18489
description:
Feasibility studies of new biotechnologies inevitably begin with laboratory-scale trials however rarely do we determine whether laboratory-scale results reflect full-scale performance or ecology. This study aims to bridge that knowledge gap using the expanded granular sludge-bed bioreactor (EGSB), a high-rate anaerobic digester type, as a model for study.Two laboratory-scale idealizations of the EGSB – a one-dimensional and a three- dimensional scale-down of a full-scale design – were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology and microbial community physiology were monitored at various depths in the sludge-beds using 16S rRNA gene sequencing (V4 region), specific methanogenic activity (SMA) assays and a range of physical and chemical monitoring methods.SMA assays indicated dominance of the hydrogenotrophic pathway at full-scale whilst a more balanced activity profile developed during the laboratory-scale trials. At each scale, Methanobacterium was the dominant methanogenic genus present. Bioreactor performance overall was better at laboratory-scale than full-scale. We observed that bioreactor idealization at laboratory-scale significantly influenced spatial distribution of microbial community physiology and taxonomy in the bioreactor sludge-bed, with 1-D bioreactor types promoting stratification of each. In the 1-D laboratory bioreactors, increased abundance of Firmicutes was associated with both granule position in the sludge bed and increased activity against acetate and ethanol as substrates. We further observed that stratification in the sludge-bed in 1-D laboratory-scale bioreactors was associated with increased richness in the underlying microbial community at species (OTU) level and improved overall performance.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJEB18489
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abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject