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Title: Myocardial infarction (MI) primes autoreactive T cells through activation of dendritic cells      
keywords:
Transcriptome or Gene expression
ID:
PRJNA374892
description:
DC subsets of the murine healthy heart consisted of IRF8-dependent conventional (c)DC1, IRF4-dependent cDC2 and monocyte-derived DCs. In steady state, cardiac self-antigen α-myosin was presented in heart-draining mediastinal lymph node (mLN) by cDC1s, driving the proliferation of antigen-specific CD4+ TCR-M T cells, and their differentiation into Tregs. Following MI, all DC subsets infiltrated the heart, while only cDCs migrated to the mLN. Here, cDC2s induced TCR-M proliferation and differentiation into IL-17/IFNγ producing effector cells. Thus, cardiac specific autoreactive T cells get activated by mature DCs following myocardial infarction. Overall design: cDC1s, cDC2s, MFs and moDCs were isolated and sorted from steady state or infarcted hearts in four independent replicates. 31 samples (RNA-Seq) in total. RNA was isolated, amplified by SMARTer amplification, converted to cDNA and then sequenced using Illumina NextSeq.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA374892
authentication:
none
authorization:
none
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject