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Title: Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate.      
keywords:
Transcriptome or Gene expression
ID:
PRJNA369681
description:
The nuclear cap-binding complex (CBC) stimulates processing reactions of capped RNAs, including their splicing, 3'-end formation, degradation, and transport. CBC effects are particular for individual RNA families, but how such selectivity is achieved remains elusive. Here, we analyze three main CBC partners known to impact different RNA species. ARS2 stimulates 3'-end formation/transcription termination of several transcript types, ZC3H18 stimulates degradation of a diverse set of RNAs, and PHAX functions in pre-small nuclear RNA/small nucleolar RNA (pre-snRNA/snoRNA) transport. Surprisingly, these proteins all bind capped RNAs without strong preferences for given transcripts, and their steady-state binding correlates poorly with their function. Despite this, PHAX and ZC3H18 compete for CBC binding and we demonstrate that this competitive binding is functionally relevant. We further show that CBC-containing complexes are short lived in vivo, and we therefore suggest that RNA fate involves the transient formation of mutually exclusive CBC complexes, which may only be consequential at particular checkpoints during RNA biogenesis. Overall design: RNA profiles of HeLa cells treated with PHAX, ZC3H18 and control siRNAs were generated by paired-end sequencing, in triplicated samples. iCLIP experiments were carried out on the CBP20, ARS2, PHAX, and ZC3H18 proteins in HeLa and HEK293 cells. Two replicates per protein were used.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA369681
authentication:
none
authorization:
none
ID:
pmid:28297668
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject