Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Expression data from three layers in the growth plate      
keywords:
Transcriptome or Gene expression
ID:
PRJNA345365
description:
The developing vertebrate growth plates in long bones have three morphologically distinct layers of chondrocytes: Round Cell layer, Flat Cell layer, and Hypertrophic Cell layer. Round chondrocytes differentiate into flat chondrocytes, and then flat chondrocytes differentiate into hypertrophic chondrocytes. To investigate the genetic programs underlying the chondrocyte differentiation, we obtained total RNA from the microdissected cryosections from each layer and then performed microarray analysis. Overall design: We euthanized 3 litters at birth to obtain biological triplicate data. Using cryosections of unfixed fresh frozen hind limbs, we manually dissected each layer on the glass slide under a dissecting microscope. We collected each piece of the sections in lysis buffer and stored at -80°C prior to RNA extraction. To prepare one sample, we dissected 18 to 20 tibiae from the same litter.
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA345365
authentication:
none
authorization:
none
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject