Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation      
keywords:
Transcriptome or Gene expression
ID:
PRJNA345341
description:
Like embryonic stem cells, induced pluripotent stem cells (iPSCs) can differentiate into all three germ layers in an in vitro system. Here, we developed a new technology for obtaining neural stem cells (NSCs) from iPSCs through chimera formation, in an in vivo environment. iPSCs contributed to the neural lineage in the chimera, which could be efficiently purified and directly cultured as NSCs in vitro. The iPSC-derived, in vivo-differentiated NSCs expressed NSC markers, and their gene-expression pattern more closely resembled that of brain-derived NSCs than in vitro-differentiated NSCs. This system could be applied for differentiating pluripotent stem cells into specialized cell type whose differentiation protocols are not well established. Overall design: Brain-NSCs (from 13.5dpc fetuses, ROSA26/OG2 heterozygous double transgenic), iPS-NSCs #1, #3 (In vitro differentiated), iPS-cNSCs (In vivo differentiated, chimeric), NED-NSCs (normal embryo-derived), MEFs (from 13.5dpc fetuses, ROSA26/OG2 heterozygous double transgenic), and ESCs (OG2 heterozygous double transgenic) were used for analysis.
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA345341
authentication:
none
authorization:
none
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject