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Title: Expression data from epidermis specific phytochrome B or global phytochrome B line comparing to phyB-9 mutant.      
keywords:
Transcriptome or Gene expression
ID:
PRJNA345317
description:
Seedling hypocotyls display negative gravitropism in the dark but agravitropism in the light. The Arabidopsis thaliana pif quadruple mutant (pifQ), which lacks four PHYTOCHROME-INTERACTING FACTORS (PIFs), is agravitropic in the dark. Endodermis-specific expression of PIF1 rescues gravitropism in pifQ mutant seedlings. Since phytochromes induce light responses by inhibiting PIFs and the COP1-SPA ubiquitin E3 ligase complex in the nucleus, we asked whether phyB can cell autonomously inhibit hypocotyl negative gravitropism in the endodermis. We found that while epidermis-specific expression of PHYB rescues hypocotyl negative gravitropism and all other phyB mutant phenotypes, endodermis-specific expression of PHYB does not. Epidermal phyB induces the phosphorylation and degradation of endodermal PIFs in response to red light. This induces a global gene expression pattern similar to that induced by red light treatment of seedlings expressing PHYB under the control of its own endogenous promoter. Our results imply that epidermal phyB generates an unidentified mobile signal that travels to the endodermis where it promotes PIF degradation and inhibits hypocotyl negative gravitropism. Overall design: ML1pro:PHYBGFP/phyB-9 and PHYBpro:PHYBGFP/phyB-9 and phyB-9 seeds were grown in continuous red condition (11.4 μmol·m−2·s−1) and then, sampled for RNA extraction. Total 4 biological replicates were used for the microarray
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA345317
authentication:
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authorization:
none
ID:
pmid:27758895
name:
Arabidopsis thaliana
ncbiID:
ncbitax:3702
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject