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Title: Impact of NCoR1 on global transcriptomic profiling in intestinal epithelial cells during development      
keywords:
Transcriptome or Gene expression
ID:
PRJNA343009
description:
To explore the molecular events engaged following the deletion of NCoR1 in intestinal tissue during development, we performed global transcriptional profiling in wild type (F/FUN) and NCoR1 intestinal deletion (ΔIECUN) mice at neonates (day 12) and adults (week 10) by utilizing RNA-seq technology. Overall design: Intestine RNA was prepared from F/FUN and DIECUN mice at either 12-day old (neonates) or 10-week old (adults) for RNA-seq. RNA from two mice were pooled and a total three samples per strain were used for RNA-seq studies. Polyadenylated mRNA was purified with the Dynabeads mRNA purification kit (ThermoFisher). The mRNA libraries were prepared for strand-specific sequencing as previously described 58and sequenced using an Illumina HiSeq 2500 by running 36 cycles. Image deconvolution, quality value calculation, and the mapping of exon reads and exon junctions were performed. Sequencing reads were aligned to the Mus musculus (UCSC mm9) genome.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA343009
authentication:
none
authorization:
none
ID:
pmid:28167773
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • P42 ES010337/ES/NIEHS NIH HHS/United States

  • R21 ES023906/ES/NIEHS NIH HHS/United States

  • R21 ES024818/ES/NIEHS NIH HHS/United States

  • T32 CA009370/CA/NCI NIH HHS/United States

  • R01 GM100481/GM/NIGMS NIH HHS/United States

  • R01 AI043477/AI/NIAID NIH HHS/United States

  • R01 GM086713/GM/NIGMS NIH HHS/United States

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