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Title: Gene expression changes in Mammary Luminal Progenitor cells after MEN1 silencing      
keywords:
Transcriptome or Gene expression
ID:
PRJNA336170
description:
Female patients with multiple endocrine neoplasia type 1 are at increased risk to develop breast cancer. We analyzed gene expression after silencing of the MEN1 gene in primary human mammary luminal progenitor cells to identify menin target genes involved in mammary tumorigenesis. Overall design: Mammary luminal progenitor cells were isolated from patient-derived normal breast tissue after tissue digestion and FACS-sorting using anitibodies directed at CD49f+ and EpCAM. After harvesting and cell sorting, cells were treated with lentiviral particles containing small hairpin RNA constructs directed against MEN1 or a control sequence and subjected to puromycin selection. Total RNA from these cells was subjected to gene expression analysis using Affymetrix Human Gene 1.0 ST Arrays. Clusters of up and down regulated genes were identified.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA336170
authentication:
none
authorization:
none
ID:
pmid:28273452
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • P01 CA080111/CA/NCI NIH HHS/United States

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