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Title: CTCF modulates Estrogen Receptor function through specific chromatin and nuclear matrix interactions [RNA-seq]      
keywords:
Transcriptome or Gene expression
ID:
PRJNA336120
description:
In our work we used high-throughput sequencing methods to get insight in the role of CTCF in ER-mediated gene regulation in luminal breast cancer cells. After assessing genome-wide binding of CTCF, ER, FOXA1 and Lamin B in MCF7 cells treated with estrogen for different time points, we could correlate the interaction to the chromatin with estrogen-induced de novo transcription (from GRO-seq data) and loop formation (from ChIA-PET, Fullwood et al., 2009). We observed that CTCF binding correlates with ER-mediated transcription and its depletion can affect ER-ER loop formation and subsequently gene expression. Overall design: RNA-seq in MCF7 cells transfected with siRNA targeting CTCF (siCTCF) or control (siNT) and treated for 0h or 3h with 100 nM Estrogen.
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA336120
authentication:
none
authorization:
none
ID:
pmid:27638884
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject

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