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Title: Global gene expression of the rodent malaria parasites Plasmodium yoelii, Plasmodium berghei and Plasmodium chabaudi blood-stage parasites      
keywords:
Transcriptome or Gene expression
ID:
PRJNA317634
description:
The purpose of this research is to identify and evaluate the global gene expression of the rodent malaria parasites Plasmodium yoelii, Plasmodium berghei and Plasmodium chabaudi blood-stage parasites and specifically compare the blood stage gene expression profiles of samples derived from previous studies on Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi Overall design: Mice were infected by intraperitoneal injections of P. berghei ANKA, P. chabaudi AS or P. yoelii 17x parasitized erythrocytes and parasitaemia and parasite stages were monitored by thin blood smears stained with Giemsa. Mice infected with P. chabaudi were highly synchronized and terminal bled every 2 hours under anesthesia over the course of 24 hr. For P. berghei and P. yoelii infection, mice were terminal bleed and the stage-specific parasitized erythrocytes were separated via Nycodenz density gradient. The ring stage interface was isolated, washed and subjected to ex vivo culture, which was then collected every 2 hr over the course of 24 hr over a complete IDC life-cycle. Samples labeled with Cy5 were hybridized against a reference RNA pool labeled with Cy3, consisting of equal amounts of P. yoelii or P. berghei or P. chabaudi RNA from each time point.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA317634
authentication:
none
authorization:
none
ID:
pmid:27322479
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject