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Title: Helicobacter pylori G27 : Helicobacter pylori NikR regulon defined by RNA-seq and ChIP-seq      
keywords:
transcriptome
ID:
PRJNA313048
description:
Helicobacter pylori is a human pathogen surviving in the prohibitive gastric environment where it can cause cancer. The ability to counteract environmental stresses and host responses is a key feature for persistence and depends on the rapid and fine-tuned expression of the genetic repertoire orchestrated by a shallow Transcriptional Regulatory Network. Metal-dependent regulators NikR and Fur are key hubs in this network; they directly control metal ion homeostasis and indirectly impact on several processes involved in H. pylori virulence and survival.In this work we combined ChIP- and RNA-sequencing to gain a comprehensive view of the NikR regulome. Strand-specific RNA-seq performed on nickel-treated or untreated cultures permitted to determine nickel-responsive transcripts, while similar experiments performed in a delta_nikR genetic background, enabled to disclose the regulatory contribution of NikR to the nickel-dependent responses. The transcriptome data were then integrated with ChIP-seq datasets in order to pinpoint the direct binding sites of NikR at high resolution, for a systematic dissection of its regulon.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA313048
authentication:
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authorization:
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name:
Helicobacter pylori G27
ncbiID:
ncbitax:563041
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject