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Title: Mus musculus : Targeted sequencing in mouse lung tumors      
keywords:
targeted loci
ID:
PRJNA310983
description:
We applied Cas9-mediated targeted mutation in mouse lung tissues, where tumors might develop. The genomic DNA was isolated from either freshly dissected lung tumours or lobes using the QIAamp DNA mini kit (Qiagen). For each target gene or potential off-target sites, a genomic region containing the target sequence was PCR-amplified using specific primers and gel purified, followed by library construction and high-throughput sequencing analysis (Illumina Hiseq 2500).
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA310983
authentication:
none
authorization:
none
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject