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Title: A RUNX2-mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells      
keywords:
Transcriptome or Gene expression
ID:
PRJNA310114
description:
The goal is to identify transcripts that are regulated by RUNX2 in SAOS2 cells. Overall design: SAOS2 cells were transduced with lentiviruses expressing shLuc, shRUNX2_3 and shRUNX2_4 for 4 days followed by 2ug/ul puromycin selection to remove untransduced cells. A no-virus control was included to monitor the transduction efficiency. These control cells died after 2 days of puromycin selection. The lentivirally transduced cells were more than 80% survived, which indicates a high transduction efficiency. After four more days in puromycin, SAOS2 cells were harvested and subjected to total RNA extraction. 1ug total RNA were used to perform RNA-seq.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA310114
authentication:
none
authorization:
none
ID:
pmid:26925584
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject