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Title: Genome-wide sequencing of hippocampal 5-hydroxymethylcytosine links plasticity genes to acute stress      
keywords:
Other
ID:
PRJNA309704
description:
We used an established chemical labeling and affinity purification method coupled with high-throughput sequencing technology to generate the first genome-wide profile of hippocampal 5hmC following exposure to acute restraint stress and a one-hour recovery. This approach found a genome-wide disruption in 5hmC associated with acute stress response, primarily in genic regions, and identified known and potentially novel stress-related targets that have a significant enrichment for neuronal ontological functions. Integration of these data with hippocampal gene expression data from these same mice found stress-related hydroxymethylation correlated to altered transcript levels and sequence motif predictions indicated that 5hmC may function by mediating transcription factor binding to these transcripts. Together, these data reveal an environmental impact on this newly discovered epigenetic mark in the brain and represent a critical step toward understanding stress-related epigenetic mechanisms that alter gene expression and can lead to the development of psychiatric disorders. Overall design: Examined the effect of a 30 minutes acute stress on hippocampal 5hmC profile of adult C57BL/6J background male mice.
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA309704
authentication:
none
authorization:
none
ID:
pmid:26598390
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • R01 HG003747/HG/NHGRI NIH HHS/United States

  • R01 NS079625/NS/NINDS NIH HHS/United States

  • T32 GM007507/GM/NIGMS NIH HHS/United States

  • U54AI117924/AI/NIAID NIH HHS/United States

  • HG007019/HG/NHGRI NIH HHS/United States

  • R01 MH102690/MH/NIMH NIH HHS/United States

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