Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Cutting Edge: IL-4, IL-21, and IFN-g Interact to Govern TBET and CD11c Expression in TLR-Activated B Cells      
keywords:
Transcriptome or Gene expression
ID:
PRJNA309547
description:
T-Box Expressed in T cells (TBET) and CD11c expression in B cells is linked with IgG2c isotype switching, virus-specific immune responses, and humoral autoimmunity. However, the activation requisites and regulatory cues governing TBET and CD11c expression remain poorly defined. In this article, we reveal a relationship among TLR engagement, IL-4, IL-21, and IFN-g that regulates TBET expression in B cells. We find that IL-21 or IFN-g directly promote TBET+ expression in the context of TLR engagement. Further, IL-4 antagonizes TBET induction. Finally, IL-21, but not IFN-g, promotes CD11c expression independent of TBET. Using influenza virus and Heligmosomoides polygyrus infections, we show that these interactions function in vivo to determine whether TBET+ and CD11c+ B cells are formed. These findings suggest that TBET+ B cells seen in health and disease share the common initiating features of TLR-driven activation within this circumscribed cytokine milieu. Overall design: Splenic B cells from replicate mice, for two different genotypes (WT and Tbx21-/-), were stimulated with either interferon-gamma (IFNG) or interleukin-21 (IL21).
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA309547
authentication:
none
authorization:
none
ID:
pmid:27430719
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • R01 AG030227/AG/NIA NIH HHS/United States

  • T32 AI055428/AI/NIAID NIH HHS/United States

  • R01 AI118691/AI/NIAID NIH HHS/United States

  • R01 AI108686/AI/NIAID NIH HHS/United States

  • K08 AI114852/AI/NIAID NIH HHS/United States

  • R01 AI113047/AI/NIAID NIH HHS/United States

  • T32 CA009171/CA/NCI NIH HHS/United States

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.