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Title: On the relationship of protein and mRNA dynamics in vertebrate embryonic development [polyA]      
keywords:
Transcriptome or Gene expression
ID:
PRJNA298254
description:
A biochemical explanation of development from the fertilized egg to the adult anatomy requires an understanding of the complement of proteins and RNAs expressed over time during embryogenesis. Here we present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most proteins change very little and duplicated genes are expressed similarly. While the correlation between mRNA levels and protein expression is poor, a mass action kinetics model parametrized by the protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides a rich resource for developmental biologists, unveiling detailed data for absolute levels of ~10K proteins and ~28K transcripts via convenient web portal. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the proteome. Overall design: mRNA from 18 samples each at a different developmental stage. Libraries were constructed using RNA enriched for mRNA by Poly(A) extraction using Dynabeads (invitrogen) and the EpiCenter ScripSeq kit V1 using 50-300ng input RNA, and 10 cycles amplification.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA298254
authentication:
none
authorization:
none
ID:
pmid:26555057
name:
Xenopus laevis
ncbiID:
ncbitax:8355
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • P40OD010997/OD/NIH HHS/United States

  • R01 HD073104/HD/NICHD NIH HHS/United States

  • R01 DK077197/DK/NIDDK NIH HHS/United States

  • R01 GM103785/GM/NIGMS NIH HHS/United States

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