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Title: DCP5, a highly conserved P-body-nucleating protein, regulates multiple small RNA-mediated silencing pathways in Arabidopsis (smRNA-seq)      
keywords:
Transcriptome or Gene expression
ID:
PRJNA143051
description:
MicroRNAs (miRNAs) and small-interfering RNAs (siRNAs) negatively regulate their targets by 1) repressing translation, 2) endonucleolytic RNA cleavage, or 3) DNA methylation resulting in transcriptional silencing. P-body/decapping components are likely required for translational repression, but are not known to function in other posttranscriptional regulatory pathways or to affect smRNA levels. Here, we show that the P-body/decapping protein DCP5 is required for miRNA-mediated translational repression but not cleavage, and to regulate the transcription of specific miRNAs. We find that this protein also affects the abundance of tRNA-derived smRNAs. Significantly, DCP5 is required for the transcriptional silencing and DNA methylation of numerous transposable/repetitive elements and imprinted genes, indicating that it is a novel component of the RNA-directed DNA methylation pathway. Our results demonstrate that DCP5 and likely the P-body itself are required for multiple smRNA-mediated silencing pathways and provide the first evidence for the spatial separation of translational inhibition and cleavage by miRNAs. Overall design: small RNA (smRNA) expression comparison between wildtype (Col-0) and dcp5 mutant plants in Arabidopsis
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA143051
authentication:
none
authorization:
none
ID:
pmid:23150631
name:
Arabidopsis thaliana
ncbiID:
ncbitax:3702
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • T32 GM007229/GM/NIGMS NIH HHS/United States

  • T32 HG000046/HG/NHGRI NIH HHS/United States

  • 5T32GM007229-37/GM/NIGMS NIH HHS/United States

  • 5T32HG000046-13/HG/NHGRI NIH HHS/United States

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