Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Comparison of total and polyribosome-associated mRNA levels in male Fmr1 KO mice and male WT littermates      
keywords:
Transcriptome or Gene expression
ID:
PRJNA142133
description:
The Fragile X Mental Retardation Protein, FMRP, is thought to regulate the translation of a specific set of neuronal mRNAs on polyribosomes. Therefore, we prepared polyribosomes on sucrose gradients and purified mRNA specifically from these fractions, as well as the total mRNA levels, to determine whether a set of mRNAs might be changed in its % association with polyribosomes in the absence of FMRP in the KO mouse model. No significant differences were found, other than the Fmr1 transcript itself, in total mRNA levels or % polyribosome association that withstood multiple test correction, in P7 Fmr1 KO mouse cerebral cortex compared with WT littermates.. Overall design: We prepared polyribosomes on sucrose gradients from 6 littermate pairs of Fmr1 KO and WT littermates (FVB background, P7 males, cerebral cortex) and purified RNA from both polyribosomal fractions and input to the gradient, reflecting total mRNA levels for comparison.
accesstypes:
download
landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA142133
authentication:
none
authorization:
none
ID:
pmid:21784246
dateReleased:
07-21-2011
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • NS40955/NS/NINDS NIH HHS/United States

  • GM46779/GM/NIGMS NIH HHS/United States

  • HD40647/HD/NICHD NIH HHS/United States

  • K99 GM095713/GM/NIGMS NIH HHS/United States

  • HD37267/HD/NICHD NIH HHS/United States

  • Howard Hughes Medical Institute/United States

  • AG30323/AG/NIA NIH HHS/United States

  • NS34389/NS/NINDS NIH HHS/United States

  • UL1 RR024143/RR/NCRR NIH HHS/United States

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.