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Title: H3K27me3 is not required for recruitment of Polycomb repressor complex 1 to target loci in mouse embryonic stem cells      
keywords:
Epigenomics
ID:
PRJNA130883
description:
The established hierarchical model explaining co-occupancy of Polycomb repressor complexes 1 and 2 (PRC 1 and 2) at target loci proposes that the chromodomain of the polycomb protein, a core PRC1 subunit, recognises the H3K27me3 histone modification catalysed by PRC2. We used chromatin immunoprecipitation to analyse PRC1 occupancy at target loci in Eed-/- mouse embryonic stem cells (ESCs) that lack H3K27me3. Occupancy of the core PRC1 proteins Ring1B and Mel18 was strongly reduced, consistent with the hierarchical model. However, levels of H2A ubiquitylation (H2AK119u1), the histone modification catalysed by PRC1, were similar to wild-type cells, suggesting PRC1 recruitment is independent of H3K27me3. ChIP-sequencing analysis of Ring1B occupancy genome wide substantiated this conclusion, demonstrating significant Ring1B levels at polycomb target loci in Eed-/- ESCs. Thus PRC1 and PRC2 are recruited independently to sites that they co-occupy. We conclude that the primary function of H3K27me3 is to increase the residency of PRC1 at target loci and thereby to contribute to the stability of PRC1 mediated silencing. Overall design: Examination of Ring1B binding in WT, Eed ko and Input of ESCs Examination of CBX7 in WT and Eed ko of ESCs
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA130883
authentication:
none
authorization:
none
ID:
pmid:22325148
dateReleased:
11-30-2011
name:
Mus musculus
ncbiID:
ncbitax:10090
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • 090532/Z/09/Z/Wellcome Trust/United Kingdom

  • 087530/Wellcome Trust/United Kingdom

  • 079249/Wellcome Trust/United Kingdom

  • G0800784/Medical Research Council/United Kingdom

  • BBS/E/B/0000H234/Biotechnology and Biological Sciences Research Council/United Kingdom

  • WT085102MA/Wellcome Trust/United Kingdom

  • G0900747 91070/Medical Research Council/United Kingdom

  • 103768/Wellcome Trust/United Kingdom

  • 081385/Wellcome Trust/United Kingdom

  • BBS/E/B/0000L748/Biotechnology and Biological Sciences Research Council/United Kingdom

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