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Title: Chromatin enrichment of TRIM24, estrogen receptor and H3K4me2 in estrogen-treated and -untreated MCF7 cells      
keywords:
Epigenomics
ID:
PRJNA130083
description:
TRIM24 PHD-Bromo domains exhibit preferential binding to unmethylated H3K4 and acetylated H3K27. TRIM24 is a co-activator of estrogen receptor (ER). The results suggest that specific ER-binding sites are depleted of H3K4me2 with estrogen treatment. TRIM24 binds these sites preferentially and facilitates ER-regulated gene expression, cell survival and proliferation. Overall design: ChIP performed on MCF7 cells +/- estrogen with antibodies against ER, TRIM24 and H3K4me2. ChIP assays of ER, co-activator TRIM24 and H3K4me2 were performed with two concentrations of antibody, without and 6h with estrogen treatment of MCF7 cells. Antibody-enriched samples were sequenced two times, and compared to an IgG negative control and Input. Enriched DNA sequenced by Illumina Solexa.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA130083
authentication:
none
authorization:
none
ID:
pmid:21164480
dateReleased:
09-17-2010
name:
Homo sapiens
ncbiID:
ncbitax:9606
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject
  • P30DK078392-01/DK/NIDDK NIH HHS/United States

  • T32 HD07325/HD/NICHD NIH HHS/United States

  • GM081627/GM/NIGMS NIH HHS/United States

  • P30 DK078392/DK/NIDDK NIH HHS/United States

  • P01 GM081627-010003/GM/NIGMS NIH HHS/United States

  • P30 EB009998/EB/NIBIB NIH HHS/United States

  • R01 GM079641/GM/NIGMS NIH HHS/United States

  • P01 GM081627-020003/GM/NIGMS NIH HHS/United States

  • UL1 TR000077/TR/NCATS NIH HHS/United States

  • U54 RR025216/RR/NCRR NIH HHS/United States

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