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Title: FoxA-a ChIP-chip on Ciona intestinalis embryo (64-cell stage)      
keywords:
Epigenomics
ID:
PRJNA123523
description:
A genomic overview of in vivo binding of a transcription factor FoxA-a in the ascidian 64-cell stage embryo. Overall design: Fertilized eggs were electroporated with a construct encoding GFP-tagged FoxA-a. After fixation, Chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE (Whole Cell Extract) DNA were amplified by LM-PCR for the Chip analysis. Two independent experiments were performed to reduce experimental errors.
accesstypes:
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA123523
authentication:
none
authorization:
none
ID:
pmid:20392745
dateReleased:
03-09-2010
name:
Ciona intestinalis
ncbiID:
ncbitax:7719
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject