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Title: Comparative transcript profiling of interaction events in Pinus sylvestris root tissues following challenge (15dpi).      
keywords:
Transcriptome or Gene expression
ID:
PRJNA104333
description:
The response mechanisms, recognition and specificity of conifer trees during interaction with pathogenic, saprotrophic or symbiotic ectomycorrhizal fungus were investigated. The roots of Pinus sylvestris were challenged for fifteen days with either Heterobasidion annosum (a pathogenic root rot fungus which attacks Norway spruce, Scots pine and broad leaf trees); Laccaria bicolor (an obligate ectomycorrhizal symbiont); or Trichoderma aureoviride (an obligate saprotroph). The gene expression data from cDNA micro-arrays consisting of 2176 Pinus taeda genes were analysed using 2-interconnected mixed linear model statistical approach. The result of the pairwise comparisons of the different treatments against un-inoculated control led to identification of genes specifically differentially expressed in the pathogenic, saprotrophic and symbiotic interactions. The results were compared with similar data obtained for two other interaction stages: 1 and 5 days post inoculation. The result of this comprehensive expression profiling will hopefully shed more light on the mechanistic basis for recognition and response of conifer trees to pathogenic and non-pathogenic fungi. Keywords: stress response Overall design: Three replicates (A,B, and C) of 12 arrays each. In each replicate each of the 4 samples is paired with each of the other 3 samples in both dye assignment possibilities (i.e. a dye-swap is performed).
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landingpage: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA104333
authentication:
none
authorization:
none
dateReleased:
07-31-2006
name:
Pinus sylvestris
ncbiID:
ncbitax:3349
abbreviation:
NCBI
homePage: http://www.ncbi.nlm.nih.gov
ID:
SCR:006472
name:
National Center for Biotechnology Information
homePage: http://www.ncbi.nlm.nih.gov/bioproject
ID:
SCR:004801
name:
NCBI BioProject