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Title: Transcription profiling by array of potato tubers with altered PME activity      
dateReleased:
12-01-2010
description:
Although processed potato tuber texture is an important trait that influences consumer preference, a detailed understanding of tuber textural properties at the molecular level is lacking. Previous work has identified tuber pectin methyl esterase activity (PME) as a potential factor impacting on textural properties and the expression of a gene encoding an isoform of PME (PEST1) was associated with cooked tuber textural properties. In this study a transgenic approach was undertaken to investigate further the impact of the PEST1 gene. Antisense and over-expressing potato lines were generated. In over-expressing lines tuber PME activity was enhanced by up to 2.3 fold whereas in antisense lines PME activity was decreased by up to 38%. PME isoform analysis indicated that the PEST1 gene encoded one isoform of PME. Analysis of cell walls from tubers from the over-expressing lines indicated that the changes in PME activity resulted in a decrease in pectin methylation. Analysis of processed tuber texture demonstrated that the reduced level of pectin methylation in the over-expressing transgenic lines was associated with a firmer processed texture. Thus there is a clear link between PME activity, pectin methylation and processed tuber textural properties.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-TABM-1062
refinement:
raw
dateSubmitted:
08-18-2010
keywords:
functional genomics
dateModified:
06-10-2011
creators:
Pete Hedley
availability:
available
types:
gene expression
name:
Solanum tuberosum
name:
strain or line
ID:
A-MEXP-1117
name:
POCI S tuberosum 44K v2
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-TABM-1062/E-TABM-1062.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-TABM-1062/E-TABM-1062.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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