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Title: Comparison of exon profiles before and after hormone induction      
dateReleased:
12-21-2010
description:
Exon array analysis component. Development, differentiation, and response to environmental stimuli are characterized by sequential changes in cellular state initiated by the de novo binding of induced or temporally regulated transcriptional factors to their cognate genomic sites. The mechanism whereby a given regulatory factor selects a limited number of in vivo targets from myriads of potential genomic binding sites is undetermined. Here we show that up to 95% of induced de novo genomic binding by the glucocorticoid receptor, a paradigmatic ligand­activated transcription factor, is targeted to pre­existing foci of accessible chromatin. Factor binding invariably potentiates chromatin accessibility. Cell­selective de novo genomic occupancy patterns appear to be comprehensively pre­determined by cell­specific differences in baseline chromatin accessibility patterns, with secondary contributions from local sequence features. The results define a novel framework for understanding regulatory factor­genome interactions, and provide a molecular basis for the tissue­selectivity of steroid pharmaceuticals and other agents that intersect the living genome. Time course of dexamethasone treatment of 2 cell types from a zero hour untreated control, 2 hour, 4 hour and 8hr treatment times. This is part of a larger study: Chromatin accessibility pre­determines de novo steroid receptor binding. There are 2 replicates per cell line per time point except in the case of 8hr Dex treated AtT-20 where a second replicate was unavailable.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-26189
refinement:
raw
alternateIdentifiers:
26189
keywords:
functional genomics
dateModified:
03-02-2012
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-AFFY-98
name:
Affymetrix GeneChip Mouse Exon 1.0 ST Array [MoEx-1_0-st-v1]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-26189/E-GEOD-26189.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-26189/E-GEOD-26189.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26189
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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