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Title: Comparative Genome Hybridization between Candida albicans and Candida dubliniensis and their derivatives      
dateReleased:
12-31-2010
description:
Total genomic DNAs of CAKS20, CdUM4b, the pseudotetraploid hybrid strain CACD1 and the progeny hybrid strain Pre3 were isolated. In an attempt to determine the number of copies of individual chromosomes, we used comparative genome hybridization (CGH) analysis using specially designed whole genome microarrays representing C. albicans and C. dubliniensis genomes. The arrays contained 60 mer probes that fell in three categories either C. albicans specific or C. dubliniensis specific or common to both. The specific probes were expected to work as internal controls when hybridized to parental genomic DNA alone. The arrays were individually hybridized to Cy3-labeled genomic DNA from either of C. albicans (CAKS20), C. dubliniensis (CdUM4b) parents or the somatic psudotetraploid hybrid strain, CACD1 and the pseudodiploid progeny hybrid Pre3. After experimentally determining species-specific probes and common probes, the ratios of intensity on the CACD1 hybrid array to those of either of the parents were calculated. The copy number was determined for the entire chromosomes by comparing individual probe intensities from the pseudotetraploid to either diploid parent genome DNA intensities. Agilent one-color CGH experiment,Organism: Human ,Agilent-24598 Genotypic designed C.albicans and C. dubliniensis Cross Species CGH Microarray 4x180k , Labeling kit: Agilent Genomic DNA labeling Kit (Part Number: 5190-0453)
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-24269
refinement:
raw
alternateIdentifiers:
24269
keywords:
functional genomics
dateModified:
11-27-2011
availability:
available
types:
gene expression
ID:
A-GEOD-10949
name:
Agilent-24598 Genotypic-designed C. albicans and C. dubliniensis Cross Species CGH Microarray 4x180k
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-24269/E-GEOD-24269.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-24269/E-GEOD-24269.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE24269
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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